Methylation sensitivity of restriction enzymes interacting with GATC sites.

924 BioTechniques Vol. 34, No. 5 (2003) inates the residual ribonuclease activity, although the effect of this treatment on the subsequent experiments should be checked. An inhibitor of RNase, such as Rnasin, can also be added to further enzymatic reactions. This study has described a degrading activity in RNA preparations from ovine, bovine, and porcine brain and ovine liver. Further experiments on these species need to be undertaken to verify whether the ribonuclease activity is present in other tissues or not. RNA extracted according to protocols other than those of Chomczynski and Sacchi should also be tested in artiodactyls as well as in species from other orders.